Study on quality standard for Humulus scandens / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3986-3990, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-310953
ABSTRACT
To control the quality of Humulus scandens, the quality standard was established in this study. According to the method recorded in the Appendix of Chinese Pharmacopoeia (2010 Edition) , the water and ash inspections were carried out. The component luteoloside and cosmosiin in Humulus scandens were identified and assayed by TLC and HPLC. The results showed a strong characteristics microscopic of Humulus scandens, and trichoromethane-methanol-formic acid (10 3 0. 3) as the mobile phase of TLC, the spots at 365 nm with a UV lamp was clear. The 16 batches of samples were analyzed by HPLC with a gradient elution of acetonitrile and phosphate solution (0.2%) at a flow rate of 1.0 mL · min(-1) and detected at 350 nm. The content of luteoloside was 0.015%- 0.651% (average 0.148%); the content of cosmosiin was 0.003%-0.118% (average 0.036%). The linear calibration curve of luteoloside and cosmosiin was acquired in the ranges of 0.011-0.364 g · L(-1) (r = 1.000 0) and 0.003-0.096 g · L(-1) (r = 1.000 0), respectively. The average recovery was 100.5% and 98.5%, respectively. The methods are convenient and reliable, which can be ap- plied for quality assessment of Humulus scandens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Controle de Qualidade
/
Padrões de Referência
/
Medicamentos de Ervas Chinesas
/
Química
/
China
/
Cromatografia Líquida de Alta Pressão
/
Humulus
Tipo de estudo:
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo
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