Generation of recombinant human tissue factor pathway inhibitor in E. coli / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 314-317, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-311045
ABSTRACT
Tissue factor pathway inhibitor (TFPI) is one of the major physiological inhibitors of the human blood coagulation cascade and may have great potential in the prevention and therapy of diseases caused by thrombus formation. In this study, recombinant human tissue factor was generated in E. coli containing a recombinant vector constructed by inserting TFPI cDNA into pGEX-2T vector. The generated recombinant TFPI (rTFPI) could be simply purified with glutathione-agarose affinity method and maintained its biological function in terms of inhibition of tissue factor and factor Xa.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fragmentos de Peptídeos
/
Farmacologia
/
Plasmídeos
/
Proteínas Recombinantes
/
Transfecção
/
Escherichia coli
/
Genética
/
Lipoproteínas
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2003
Tipo de documento:
Artigo
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