Serum cytokine profiles in stable survivors with clinical liver transplantation / 中华外科杂志
Chinese Journal of Surgery
;
(12): 207-209, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-311121
ABSTRACT
<p><b>OBJECTIVE</b>To elucidate the profile of serum cytokines and adhesion molecules in stable survivors with clinical liver transplantation.</p><p><b>METHODS</b>Flow cytometric analysis was used to analyse the phenotype of T cell subsets in peripheral blood mononuclear cells (PBMCs) from group of liver transplantation (LTx) (n = 22), primary liver carcinoma (PLC) (n = 13) and healthy control (n = 12). Enzyme-linked immunoabsorbent assay (ELISA) was used to determine the serum cytokines and adhesion molecules profiles in stable survivors with clinical liver transplantation.</p><p><b>RESULTS</b>Percentage of CD3(+) T cell and CD8(+) T cell, as well as ratio of CD4(+) to CD8(+) revealed no difference among three groups. The percentage of CD3(+)CD25(+) T cells in LTx group was found higher than that in healthy group (P = 0.022). Th1 cytokines (IL-2, IFN-gamma) and Th2 cytokines (IL-4, IL-10), as well as TNF-alpha displayed no significant difference among three groups. The levels of IL-6, ICAM-1 and P-selectin in serum were not found any difference between LTx group and PLC group, while the levels of IL-6, ICAM-1 and P-selectin in serum shown significant difference between LTx and healthy groups (P = 0.048, 0.000 and 0.025, respectively).</p><p><b>CONCLUSIONS</b>Our data demonstrates that effector T-cells can also be activated and exert immunoresponse to grafts permanently under the treatment of immunosuppressant. Adhesion molecules (ICAM-1, P-Selectin) and pro-inflammatory cytokines (IL-6, TNF-alpha) might be involved in the process of chronic graft damage induced by allo-immunoresponse.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Terapêutica
/
Sangue
/
Ensaio de Imunoadsorção Enzimática
/
Linfócitos T
/
Citocinas
/
Interleucina-4
/
Interleucina-6
/
Transplante de Fígado
/
Interleucina-2
/
Fator de Necrose Tumoral alfa
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2004
Tipo de documento:
Artigo
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