The role of the immunohistochemistry for hMLH1 and hMSH2 with detection of microsatellite instability to identify the kindreds with hereditary nonpolyposis colorectal cancer / 中华外科杂志
Chinese Journal of Surgery
;
(12): 809-811, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-311203
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the specificity and sensitivity of the immunohistochemistry for hMLH1 and hMSH2 with detection of microsatellite instability (MSI) to identify the kindreds with hereditary nonpolyposis colorectal cancer and to analyse its value in clinical practice.</p><p><b>METHOD</b>Specimens of 16 cases with HNPCC and 16 cases with sporadic colorectal cancer were detected by immunostaining with hMLH1 and hMSH2 and MSI was also detected.</p><p><b>RESULTS</b>The specificity and sensitivity of the immunohistochemistry for hMLH1 and hMSH2 were 91.7% and 87.5% respectively. The specificity and sensitivity of MSI were 100% and 75.0%. By combining two methods, the specificity and sensitivity were 91.7% and 93.8% respectively.</p><p><b>CONCLUSIONS</b>By combination of the immunohistochemistry for hMLH1 and hMSH2 and detection of MSI to identify the kindreds with HNPCC, the specificity and sensitivity are improved which is better than to use either of them alone. And it is very easy and cheap that it can be used in clinics.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Imuno-Histoquímica
/
Proteínas Nucleares
/
Proteínas de Transporte
/
Neoplasias Colorretais Hereditárias sem Polipose
/
Proteínas Proto-Oncogênicas
/
Sensibilidade e Especificidade
/
Repetições de Microssatélites
/
Instabilidade Genômica
/
Proteínas Adaptadoras de Transdução de Sinal
/
Proteínas de Ligação a DNA
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2003
Tipo de documento:
Artigo
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