Detection of DNA damage of workers occupationally exposed to lead with flow cytometer assay / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 266-269, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-311245
ABSTRACT
<p><b>OBJECTIVE</b>To study DNA damage of workers occupationally exposed to lead with flow cytometer assay.</p><p><b>METHODS</b>The lymphocytes were obtained from 41 workers occupationally exposed to lead (comparable group) and another 50 from control group. Flow cytometer (FCM) assay was used to detect DNA damage.</p><p><b>RESULTS</b>DNA damage rate and geometric mean fluorescence intensity in the comparable group were significantly higher than those in the control group (P<0.05). There were no significant differences in the DNA damage and geometric mean fluorescence intensity between different age groups (P>0.05). The differences in correlation analysis between blood lead, urine lead, delta-ALA and DNA damage rate were not significant (P>0.05). The correlation analysis showed no statistical significance between concentration of blood lead, urine lead, delta-ALA and geometric mean fluorescence intensity (P>0.05). There was positive correlations not only between the high concentration of blood lead, delta-ALA and damage rate of DNA, but also between the high concentration of blood lead and geometric mean fluorescence intensity. The coefficient r showed statistical significance (P<0.05).</p><p><b>CONCLUSION</b>Occupational lead exposure can cause DNA damage. Gamma H2AX flow cytometer assay is a sensitive, objective and effective method for detection of DNA damage of peripheral blood lymphocytes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Dano ao DNA
/
Linfócitos
/
Exposição Ocupacional
/
Citometria de Fluxo
/
Chumbo
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Ano de publicação:
2009
Tipo de documento:
Artigo
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