Effect of CDK Inhibitor LS-007 on Acute Lymphoblastic Leukemia and Its Mechanism / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of cyclin dependent kinase(CDK) inhibitor LS-007 on acute lymphoblastic leukemia and its mechanism.</p><p><b>METHODS</b>The acute lymphocytic leukemia cell line was cultured and treated by LS-007, flavopiridol and ABT-199, then the changes of apoptosis-related factor mRNA and protein levels were detected by using mRNA quantitative PCR and Werstern blot.</p><p><b>RESULTS</b>quantitative PCR and Western blot detection showed that the levels of antiapoptotic protein decreased significantly in acute lymphoblastic leukemia cells after LS-007 treatment, and the pro-apoptotic effect of LS-007 combined with ABT-199 was much better.</p><p><b>CONCLUSION</b>LS-007 can affect the phosphorylation of RNA polymerase sites and promote cell apoptosis through changing the activities of CDK, thus having some positive significance for relieving acute lymphoblastic leukemia.</p>