Expression of Death Associated Protein Kinase 1 in Chronic Lymphocytic Leukemia / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of death-associated protein kinase 1(DAPK1) in chronic lymphocytic leukemia(CLL).</p><p><b>METHODS</b>The DAPK1 expression was studied by means of MEC1 cells and B lymphocytes from blood samples of the patients with CLL. The quantitative detection of mRNA and Western blot were used to detecte the expression of DAPK1 and autophagy-related genes at both mRNA and protein levels.</p><p><b>RESULTS</b>mRNA quantitative detection and Western blot displayed that the DAPK1 expression in the patients with CLL was silenced. So, the expression of DAPK1 and autophagy related genes in MEC1 cells was not significantly different, no matter the cells were treated with or without INF-γ.</p><p><b>CONCLUSION</b>Scilencing of DAPK1 expression in CLL results in abnormality of autophagy behavior, thus leading to the occurence of disease.</p>