Your browser doesn't support javascript.
loading
Retroviral endostatin gene transfer inhibits human colon cancer cell growth in vivo / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1582-1584, 2003.
Artigo em Inglês | WPRIM | ID: wpr-311627
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic effect of retroviral endostatin gene transfer on the human colon cancer cell line, LoVo.</p><p><b>METHODS</b>A retroviral vector pLESSN expressing secretable endostatin was constructed and packaged with a titer of 8.2 x 10(5) CFU/ml. A LoVo cell line was subjected to retrovirus-mediated endostatin gene transfer. The proviral integration of endostatin was analyzed with PCR. The function of endostatin was tested by MTT assay in vitro and a mouse xenograft model in vivo.</p><p><b>RESULTS</b>After transfection and superinfection, amphotropic retrovirus was collected, and transduction with amphotropic retroviruses resulted in endostatin proviral integration. The endostatin secreted by transduced LoVo cells markedly inhibited cell growth up to 67% (P<0.001), compared with the control cells. The gene expression of endostatin in LoVo colon tumor cells significantly inhibited tumor growth in vivo. There was an 86% reduction in tumor size in the endostatin-transduced group, accompanied by a reduction in vessels, compared with the control group (P<0.01).</p><p><b>CONCLUSION</b>Retroviruses can allow functional expression of the endostatin gene in human colon tumors, showing promise for an antitumor strategy using antiangiogenesis.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Retroviridae / Terapêutica / Divisão Celular / Neoplasias do Colo / Técnicas de Transferência de Genes / Linhagem Celular Tumoral / Endostatinas / Vetores Genéticos / Genética Limite: Humanos Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2003 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Retroviridae / Terapêutica / Divisão Celular / Neoplasias do Colo / Técnicas de Transferência de Genes / Linhagem Celular Tumoral / Endostatinas / Vetores Genéticos / Genética Limite: Humanos Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2003 Tipo de documento: Artigo