Capillary zone electrophoresis for determination of thymulin in human plasma / 药学学报
Acta Pharmaceutica Sinica
;
(12): 214-216, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-312007
ABSTRACT
<p><b>AIM</b>To establish a capillary zone electrophoresis (CZE) method for determination of thymulin in human blood plasma. Thymulin is a nonapeptide produced by the thymic epithelium.</p><p><b>METHODS</b>The uncoated capillary used was 75 microns ID x 57 cm (50 cm effective length). The experimental conditions were as follows the running buffer was phosphate-borate buffer containing 50 mmol.L-1 Na2B4O7-10 mmol.L-1 Na2HPO4 (pH 9.10); running voltage was 12 kV; operated temperature was 25 degrees C; running time was 13 minutes, detective wavelength was 200 nm.</p><p><b>RESULTS</b>The linear range of the thymulin standard curve was 1-10 micrograms.mL-1, gamma = 0.9990, the average recovery was 70.97% with RSD of 3.32%, the RSD of reproducibility was 3.05%.</p><p><b>CONCLUSION</b>The method of CZE for determination of thymulin in human plasma is simple and reliable.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasma
/
Fator Tímico Circulante
/
Química
/
Eletroforese Capilar
Limite:
Criança, pré-escolar
/
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2002
Tipo de documento:
Artigo
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