Determination of six effective components in Rheum by cyclodextrin modified micellar electrokinetic chromatography / 药学学报
Acta Pharmaceutica Sinica
;
(12): 798-801, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-312046
ABSTRACT
<p><b>AIM</b>To determine six effective components (aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol) in Rheum.</p><p><b>METHODS</b>Using buffer solution containing 20 mmol.L-1 borax, 20 mmol.L-1 sodium deoxycholate (SDC), 20 mmol.L-1 sodium taurocholate (STC), 15 mmol.L-1 beta-cyclodextrin (beta-CD) and O-phthalic acid as the internal standard, the six components were determined by cyclodextrin modified micellar electrokinetic chromatography.</p><p><b>RESULTS</b>In less than 25 min, aloe-emodin, rhein, emodin, rhaponticin, physion and chrysophanol were separated. The separation conditions were optimized by adjusting buffer pH, concentrations of SDC, STC and beta-CD. The linearity ranges of aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 4-34, 5-40, 4-60, 5-80, 6-90 and 5-85 micrograms.mL-1 respectively. Relative standard deviation (RSD) of the method was less than 2.2%. The recoveries of aloe-emodin, rhein, emodin, rhaponticin, physcion and chrysophanol were 100.0%, 98.3%, 100.4%, 94.6%. 95.2% and 93.8% respectively. Raw Rheum, Mongolian Rheum and Rheum tanguticum samples were analyzed.</p><p><b>CONCLUSION</b>This method can be an effective one for identification of Rhubarb.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Controle de Qualidade
/
Rheum
/
Estilbenos
/
Química
/
Antraquinonas
/
Emodina
/
Cromatografia Capilar Eletrocinética Micelar
/
Ciclodextrinas
/
Métodos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2002
Tipo de documento:
Artigo
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