Hepatitis B virus X protein up-regulates tumor necrosis factor-α expression in cultured mesangial cells via ERKs and NF-κB pathways
Asian Pacific Journal of Tropical Biomedicine
;
(12): 217-222, 2013.
Artigo
em Inglês
| WPRIM
| ID: wpr-312425
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of hepatitis B virus (HBV) X protein (HBx) on the expression of tumor necrosis factor-α (TNF-α) in glomerular mesangial cells (GMCs) and the underlying intracellular signal pathways.</p><p><b>METHODS</b>The plasmid pCI-neo-X that carries the X gene of hepatitis B virus was transfected into cultured GMCs. HBx expression in the transfected GMCs was assessed by Western-blot. TNF-α protein and mRNA were assessed by ELISA and semi-quantitative RT-PCR, respectively. Three kinase inhibitors-U0126, an inhibitor of extracellular signal-regulated kinases (ERKs); lactacystin, an inhibitor of nuclear factor-κB (NF-κB); and SB203580, a selective inhibitor of p38 MAP kinase (p38 MAPK) were used to determine which intracellular signal pathways may underlie the action of HBx on TNF-α expression in transfected GMCs.</p><p><b>RESULTS</b>A significant increase in HBx expression in pCI-neo-X transfected GMCs was detected at 36 h and 48 h, which was not affected by any of those kinase inhibitors mentioned above. A similar increase in the expression of both TNF-α protein and mRNA was also observed at 36 h and 48 h, which was significantly decreased in the presence of U0126 or lactacytin, but not SB203580.</p><p><b>CONCLUSIONS</b>HBx upregulates TNF-α expression in cultured GMCs, possibly through ERKs and NF-κB pathway, but not p38 MAPK pathway.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Ensaio de Imunoadsorção Enzimática
/
Transdução de Sinais
/
Transativadores
/
Regulação da Expressão Gênica
/
Vírus da Hepatite B
/
Fator de Necrose Tumoral alfa
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Células Mesangiais
/
Genética
Limite:
Humanos
Idioma:
Inglês
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2013
Tipo de documento:
Artigo
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