The influence of lipopolysaccharide on adipose metabolism in liver during shock stage of scalded rats / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the influence of lipopolysaccharide (LPS) on adipose metabolism in liver during shock stage of scalded rats.</p><p><b>METHODS</b>Sixty adult Wistar rats were inflicted with 30% TBSA full thickness scald and were randomly divided into 3 groups i. e. sham group (control, n = 20), simple scald group [(n = 20) and LPS group (n = 20, with intra-peritoneal injection of 3.0 mg/kg LPS at 2 postscald hour (PSH)]. The contents of LPS, tumor necrosis factor alpha (TNF-alpha), free fatty acids (FFA) in plasma and adenosine triphosphate (ATP), triglyceride (TG), malonaldehyde (MDA) in liver in each group were determined at 24 and 48 PSH. The histological changes in hepatic tissue in each group were also observed.</p><p><b>RESULTS</b>The plasma contents of FFA in LPS group at 24 and 48 PSH were 2.3 +/- 0.3 mmol/L and 2.5 +/- 0.4 mmol/L, respectively, which were obviously higher than those in control (0.4 +/- 0.3 mmol/L, 0.5 +/- 0.3 mmol/L) and scald (0.9 +/- 0.3, 1.2 +/- 0.5 mmol/L, P <0.01) groups. Meanwhile, there was obvious difference in the contents of TG and ATP in liver between LPS group (TG 530 +/- 30 mmol/g, ATP 1.7 +/- 0.5 micromol/g) and scald group (TG 242 +/- 27 mmol/g, ATP 6.0 +/- 2.4 micromol/g, P < 0.01). Pathological examination revealed that adipose denaturalization and injury to mitochondria in hepatocytes in scald group were significantly milder than those in LPS group. The morphology of hepatocyte in control group appeared normal.</p><p><b>CONCLUSION</b>LPS challenge to burn subjects could induce impairment in utilizing fat derived energy, and it would aggravate adipose denaturalization in the liver.</p>