miR-128a is up-regulated in hepatocellular carcinoma and promotes tumor cell proliferation by targeting RND3 / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of microRNA-128a (miR-128a) and its role in hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>Nineteen pairs of fresh surgical specimens of HCC and adjacent tissues were examined for miR-128a expression using qRT-PCR. A miR-128a mimics or inhibitor was transfected into HCC cells, and the cell viability was analyzed by MTT assay. RND3, one of the potential targets of miR-128a, was predicted by bioinformatics software and demonstrated by dual luciferase reporter assay. The expression of RND3 after transfection was detected using qRT-PCR and Western blotting, and the cell cycle-related proteins were determined with Western blotting.</p><p><b>RESULTS</b>The expression of miR-128a were significantly up-regulated in HCC tissues as compared to the adjacent tissues (P<0.05). In cultured HCC cells, miR-128a promoted the cell proliferation and resulted in down-regulated RND3 mRNA and protein expressions by targeting RND3' 3'UTR (P<0.05) and also in the down-regulation of cyclin B1, cyclin D1 and CDK4 protein expressions.</p><p><b>CONCLUSION</b>miR-128a is up-regulated in HCC and promotes HCC cell proliferation by targeting RND3.</p>