Alternation of retinal complement system in a mouse model of lipopolysaccharide- induced uveitis / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the activation of the complement system in the retina in a mouse model of endotoxin-induced uveitis (EIU).</p><p><b>METHODS</b>Balb/c mice were randomly divided into control group and lipopolysaccharide (LPS)-induced EIU group. Twenty-four hours after modeling, the expressions of the major complement components of the classical pathway (CP), mannose-binding lectin (MBL) pathway, alternative pathway (AP), and terminal pathway in the retina were determined by quantitative RT-PCR. Western blotting was employed to examine the protein expressions of the key components of the complement system involved in the CP and AP pathways in the retina.</p><p><b>RESULTS</b>s Normal mouse retina expressed a variety of complement components that were involved mainly in the CP and AP pathways. The expressions of the complement components involved in the CP and AP pathways were up-regulated in the retina of mice with EIU. Although MASP1 and MASP2 were detected in the retina in both the EIU and control groups, their expressions were weak and showed no significant difference between the two groups.</p><p><b>CONCLUSION</b>The AP and CP but not the MBL pathways of the complement system are activated in the retina of mice with EIU, suggesting a role of the activated complement system in the pathological process of EIU.</p>