Establishment of urinary proteomic map on two-dimensional polyacrylamide gel electrophoresis in children with idiopathic nephrotic syndrome / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 122-124, 2007.
Article
em Zh
| WPRIM
| ID: wpr-312761
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a urinary proteomic map on two-dimensional polyacrylamide gelelectrophoresis (2-DE) in children with idiopathic nephrotic syndrome (INS).</p><p><b>METHODS</b>The proteins from INS children were purified by four various means, separated by 2-DEand stained by silver.</p><p><b>RESULTS</b>The sequential preparation of urinary proteins by acetone precipitation and dislysis, when the sample was 300 microg, resulted in a clear background, well-resolved and reproducible 2-DE urinary protemic map in children with INS.</p><p><b>CONCLUSIONS</b>A steady 2-DE technique for urinary protemic map in children with INS was established, which can be effectively applied in urinary proteomics of the disease.</p>
Texto completo:
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Índice:
WPRIM
Assunto principal:
Proteinúria
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Urina
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Eletroforese em Gel Bidimensional
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Proteômica
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Métodos
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Síndrome Nefrótica
Limite:
Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2007
Tipo de documento:
Article