The quantity analysis of reverse transcriptase in porcine endogenous retrovirus expressed in Banna minipig inbred / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 594-596, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-312920
ABSTRACT
Quantitative RT(reverse transcriptase) assay was established to detect the reverse transcriptase in plasma of thirty-four Chinese Banna minipig inbred in this work. The protocol was given in the RT kit (Roche), using HIV-1 as the positive control of the kit and supernatant of PK-15 as the PERV positive control respectively. The results show that positive reverse transcriptase reaction can be detected in the plasma of the pigs, but the levels are much lower than that of HIV-1 and lower than that of PERV in supernatant of PK-15.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Porco Miniatura
/
Virologia
/
Sangue
/
DNA Polimerase Dirigida por RNA
/
Animais Endogâmicos
/
Retrovirus Endógenos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2003
Tipo de documento:
Artigo
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