FHIT gene is abnormal in tongue carcinoma cell line / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 472-475, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-312952
ABSTRACT
To study the alteration of FHIT gene in tongue carcinoma Tca8113 cell line, total RNA of Tca8113 cells was extracted. The transcript of the FHIT gene of the Tca8113 cell line was detected with nest RT-PCR, and DNA was sequenced. The result showed that abnormal transcript (about 247 bp) of FHIT gene was detected in the Tca8113 cell line. The sequence analysis of the aberrant cDNAs revealed deletions of exons 1-8. Therefore, the deletion of the FHIT gene in Tca8113 cell line might support the hypothesis that the FHIT gene alteration is involved in the development of tongue carcinoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias da Língua
/
Dados de Sequência Molecular
/
Células Tumorais Cultivadas
/
Sequência de Bases
/
Genes Supressores de Tumor
/
Análise de Sequência de DNA
/
Deleção de Genes
/
Hidrolases Anidrido Ácido
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2003
Tipo de documento:
Artigo
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