Effects of cyclooxygenase-2 antisense vector on proliferation and sensitivity to cisplatin of H1299 cells / 中国肺癌杂志
Chinese Journal of Lung Cancer
; (12): 424-427, 2005.
Article
em Zh
| WPRIM
| ID: wpr-313330
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WPRO
ABSTRACT
<p><b>BACKGROUND</b>At present, it has been known that cyclooxygenase-2 (COX-2) plays a crucial role in invasion, development and metastasis of non-small cell lung cancer (NSCLC). In order to explore whether the expression of COX-2 inhibits the occurrence and development of NSCLC, antisense vector of human COX-2 gene is transfected into COX-2 highly expressing NSCLC cell line H1299 and its effects on proliferation and sensitivity to cisplatin of H1299 are analysed.</p><p><b>METHODS</b>H1299 cells were transfected with antisense vector of human COX-2 gene using LipoVecTM transfecting technique. Transfected cells were selected with Geneticin (G418). The COX-2 mRNA level was examined by using reverse polymerase chain reaction (RT-PCR). The COX-2 protein level was examined by Western Blot. The proliferative status and sensitivity to cisplatin of cells was measured by methabenzthiazuron (MTT) assay.</p><p><b>RESULTS</b>RT-PCR showed a lower COX-2 mRNA level in transfected cells. The level of COX-2 protein was decreased apparently. The proliferative index of the transfected cells decreased significantly (P < 0.05). The IC50 value of cisplatin decreased remarkably in transfected cells (1.8mg/l) compared with that in H1299 cells without transfection (3.8mg/l) (P < 0.05).</p><p><b>CONCLUSIONS</b>Transfection with antisense vector of human COX-2 gene can not only inhibit the proliferation of H1299 cells, but also increase the sensitivity to cisplatin of H1299 cells.</p>
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WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Lung Cancer
Ano de publicação:
2005
Tipo de documento:
Article