Expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 805-808, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-313890
ABSTRACT
This study was aimed to investigate the expression of radioresistant genes survivin and HO-1 in mesenchymal stem cells (MSC). Human bone marrow MSC were isolated and enriched by Fircoll density gradient centrifugation, then identified by flow cytometry. MSC were induced with dexamethasone, insulin, 3-isobutyl-1-methyl-xanthine (IBMX) and indomethacin to differentiate into adipocytes. Then the expression of survivin and HO-1 in MSC was detected by RT-PCR. The results indicated that the expressions of surface antigen CD34 and HLA-DR in MSC in vitro were negative while the expressions of CD44 and CD71 were positive. MSC could be differentiated into adipocytes by inductor. RT-PCR showed the expression of radioresistant genes survivin and HO-1 in MSC. It is concluded that MSC have lower sensitivity to radiation, which may associate with the expression of radioresistant genes survivin and HO-1 in MSC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Efeitos da Radiação
/
Células da Medula Óssea
/
Expressão Gênica
/
Diferenciação Celular
/
Células Cultivadas
/
Heme Oxigenase-1
/
Proteínas Inibidoras de Apoptose
/
Células-Tronco Mesenquimais
/
Citometria de Fluxo
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo
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