Effect of different concentrations of bortezomib on the expression of ERK, JNK and P38 in daunorubicin-resistant K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 671-675, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-313919
ABSTRACT
The aim of this study was to investigate the effect of proteasome inhibitor bortezomib on the expression of ERK, JNK, and P38 in daunorubicin (DNR)-resistant K562 cells and its mechanism. MTT method was used to determine the drug-resistant K562 cells and the cellular toxicity of bortezomib; Western blot was used to detect the expression of protein ERK, JNK and P38 in K562 cells after treatment with 100 nmol/L DNR alone or combined with 1 nmol/L and 10 nmol/L bortezomib for 36 hours. Flow cytometry assay was used to detect the apoptosis rate in each group cells. The results indicated that the expression of ERK and P38 were significantly suppressed (p < 0.05) and the expression of JNK was significantly enhanced (p < 0.05) in the cells treated by DNR combined with bortezomib. It is concluded that bortezomib can decrease the expressions of protein ERK and P38 and enhance the expression of JNK, the bortezomib reverses the cellular drug-resistance and promote cell apoptosis through MAPK pathway.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Inibidores de Proteases
/
Pirazinas
/
Ácidos Borônicos
/
Resistencia a Medicamentos Antineoplásicos
/
Células K562
/
Proteínas Quinases JNK Ativadas por Mitógeno
/
Proteínas Quinases p38 Ativadas por Mitógeno
/
Bortezomib
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo
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