Growth inhibition and apoptosis inducing mechanisms of curcumin on human ovarian cancer cell line A2780 / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 126-131, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-314073
ABSTRACT
<p><b>OBJECTIVE</b>To explore the growth inhibition effects and apoptosis inducing mechanisms of curcumin on human ovarian cancer cell line A2780.</p><p><b>METHODS</b>After treatment with 10 - 50 micromol/L curcumin for 6 - 24 h, the growth activity of A2780 cancer cells were studied by [4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) colorimetry. Cellular apoptosis was inspected by flow cytometery and acridine orange-ethidium bromide fluorescent staining methods. The fragmentation of cellular chromosome DNA was detected by DNA ladder, the ultrastructural change was observed under a transmission electron microscope, and the protein levels of nuclear factor-kappa B (NF-kappaB, P65) and cysteinyl aspartate specific protease-3 (Caspase-3) in ovarian cancer cells were measured by immunohistochemistry.</p><p><b>RESULTS</b>After treatment with various concentrations of curcumin, the growth inhibition rates of cancer cells reached 62.05% - 89.24%, with sub-G(1) peaks appearing on histogram. Part of the cancer cells showed characteristic morphological changes of apoptosis under fluorescence and electron microscopes, and the rate of apoptosis was 21.5% - 33.5%. The protein expression of NF-kappaB was decreased, while that of Caspase-3 was increased in a time-dependent manner.</p><p><b>CONCLUSION</b>Curcumin could significantly inhibit the growth of human ovarian cancer cells; inducing apoptosis through up-regulating Caspase-3 and down-regulating gene expression of NF-kappaB is probably one of its molecular mechanisms.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Patologia
/
Farmacologia
/
Laranja de Acridina
/
Imuno-Histoquímica
/
Regulação para Baixo
/
Divisão Celular
/
Regulação para Cima
/
NF-kappa B
/
Apoptose
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Chinese journal of integrative medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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