Effect of low-dose 2-methoxyestradiol on the expressions of two isoforms of PRDM1 gene in myeloma / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of low-dose 2-methoxyestradiol (2ME2) on the expression of two isoforms of PRDM1 gene (PRDM1alpha with and PRDM1beta without PR-domain) during the myeloma cell differentiation.</p><p><b>METHODS</b>In myeloma cell line NCI-H929, RPMI8226, KM3 and LP-1, PRDMla and PRDM1beta transcripts were detected by real-time quantitative PCR after treatment with low-dose of 2ME2 (0.5 micromol/L) for 0 h, 48 h and 72 h.</p><p><b>RESULTS</b>Both PRDM1alpha and PRDMIbeta3 were time-dependently upregulated, and the PRDMlalpha/beta ratio was elevated from 1.461 +/- 0.033 to 2.663 +/- 0.381 (P < 0.01), 1.929 +/- 0.334 to 2.727 +/- 0.362 (P < 0.05), 1.471 +/- 0.012 to 4.367 +/- 0.243 (P < 0.01) and 1.660 +/- 0.042 to 3.059 +/- 0.167 (P < 0.01) in NCI-H929, RPMI8226, KM3 and LP-1 cells respectively.</p><p><b>CONCLUSION</b>Low dose of 2ME2 can induce differentiation of myeloma cells as well as up-regulate the expression of PRDMlalpha and PRDM1beta in these cells. Elevation of PRDM1alpha/beta ratio was in a time-dependent manner and positively associated with cell differentiation and in accordance with Ying-Yang mechanism of PR-domain-containing gene family.</p>