Simultaneous determination of rosmarinic acid, oridonin and chrysoplenetin in Isodon rubescens by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2343-2347, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-315028
ABSTRACT
An HPLC method was developed for simultaneous quantitation of rosmarinic acid, oridonin and chrysoplenetin in the aerial parts of Isodon rubescens. Samples were analyzed on an Ultimate C18 column (4.6 mm x 250 mm, 5 microm) with methanol and water containing 0.1% formic acid as mobile phases in a linear gradient mode. The flow rate was 1.0 mL x min(-1) and the temperature was set at 30 degrees C. The PDA detector wavelengths were set at 338 nm for rosmarinic and chrysoplenetin and at 242 nm for oridonin. The linear ranges were 0.222-2.78, 0.227-2.84 and 0.005-0.071 microg for rosmarinic acid, oridonin and chrysoplenetin, respectively. The average recoveries of the three constituents were 102.9% (RSD 1.9%), 99.6% (RSD 1.1%) and 102.5% (RSD 0.94%), respectively. This method was proved to be accurate and repeatable, and can be used for quality control of the aerial parts of I. rubescens.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Flavonoides
/
Medicamentos de Ervas Chinesas
/
Extratos Vegetais
/
Química
/
Cromatografia Líquida de Alta Pressão
/
Cinamatos
/
Isodon
/
Diterpenos do Tipo Caurano
/
Depsídeos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo
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