Effects of heparanase inhibition by RNA interference on proliferation, invasiveness and apoptosis of lung cancer cells / 中华病理学杂志
Chinese Journal of Pathology
; (12): 826-830, 2008.
Article
em Zh
| WPRIM
| ID: wpr-315060
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of heparanase expression inhibition on the proliferation, invasiveness and apoptosis of human lung adenocarcinoma cell line A549 cells.</p><p><b>METHODS</b>Recombinant eukaryotic expression plasmid pshRNA-Hpa targeting human heparanase gene was constructed. A549 cells were cultured in DMEM and transfected with pshRNA-Hpa. The expression of heparanase mRNA and protein were examined by RT-PCR and Western blot. The proliferation, invasiveness and apoptotic rates of A549 cells were determined by MTT method, matrigel invasion assays and flow cytometry respectively.</p><p><b>RESULTS</b>The expression levels of heparanase mRNA and protein were down-regulated in A549 transfected with pshRNA-Hpa. The number of cells penetrating matrigel and the proliferation ability of A549 cells transfected with pshRNA-Hpa were reduced significantly compared to the control cells. The apoptotic rate of A549 cells transfected with pshRNA-Hpa was 12.53% +/- 0.34%, being significantly higher than that of the control cells (both P < 0.01). Western-blot showed that inhibition of heparanase expression led to reduced Akt phosphorylation.</p><p><b>CONCLUSIONS</b>The recombinant plasmid pshRNA-Hpa effectively inhibited the expression of heparanase, thus suppressing the proliferation and invasion and inducing apoptosis of A549 cells. The effects may be due to the down-regulation of Akt phosphorylation level.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Patologia
/
Farmacologia
/
RNA Mensageiro
/
Transfecção
/
Adenocarcinoma
/
Regulação para Baixo
/
Apoptose
/
Carcinoma Pulmonar de Células não Pequenas
/
RNA Interferente Pequeno
/
Interferência de RNA
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Ano de publicação:
2008
Tipo de documento:
Article