SiRNA inhibition of E6AP expression in cervical cancer cells / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 822-825, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-315061
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of RNA interference (RNAi) targeting E6AP on the proliferation and apoptosis of HeLa cells.</p><p><b>METHODS</b>HeLa cells were cultured and divided into 3 groups blank control group, cells transfected with nonsense siRNA (small interference RNA), and cells transfected with specific E6AP siRNA. The expressions of E6AP mRNA and protein were detected by RT-PCR and Western blot before and after the transfection respectively. Cell proliferation was determined by methylthiazolyl tetrazolium (MTT). The cell apoptosis index was assessed by flow cytometry.</p><p><b>RESULTS</b>Upon treatment with E6AP siRNA for 24, 48 and 72 h, the expression level of E6AP mRNA decreased 33%, 72% and 70% than siRNA treated group. The protein expression levels in 48 h and 72 h E6AP siRNA groups decreased 38%, 59% comparing with those of the nonsense siRNA treated group (P < 0.05). The proliferative capacity of cells transfectd with E6AP siRNA was significantly lower than blank control group (F = 101.38, P < 0.05) and siRNA treated group (F = 38.64, P < 0.05). The apoptosis index of HeLa cells treated with E6AP siRNA was significantly higher than that of the nonsense siRNA (F = 41.48, P < 0.05) and the blank control group (F = 86.36, P < 0.05).</p><p><b>CONCLUSION</b>SiRNA targeting can effectively suppress the expression levels of E6AP mRNA, corresponding with a proliferation inhibition and an enhanced apoptosis of HeLa cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Células HeLa
/
Regulação Neoplásica da Expressão Gênica
/
Neoplasias do Colo do Útero
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Inativação Gênica
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Linhagem Celular Tumoral
/
Ubiquitina-Proteína Ligases
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS