Expression of Total Vascular Endothelial Growth Factor and the Anti-angiogenic VEGF 165 b Isoform in the Vitreous of Patients with Retinopathy of Prematurity / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2505-2509, 2015.
Artigo
em Inglês
| WPRIM
| ID: wpr-315306
ABSTRACT
<p><b>BACKGROUND</b>This study was to examine the expression of total vascular endothelial growth factor (VEGF) and the anti-angiogenic VEGF 165 b isoform in the vitreous body of retinopathy of prematurity (ROP) patients, and to further study the role of the VEGF splicing in the development of ROP.</p><p><b>METHODS</b>This was a prospective clinical laboratory investigation study. All patients enrolled received standard ophthalmic examination with stage 4 ROP that required vitrectomy to collect the vitreous samples. The control samples were from congenital cataract patients. The expression of total VEGF and the anti-angiogenic VEGF 165 b were measured by enzyme-linked immunosorbent assay. Results were analyzed statistically using nonparametric tests.</p><p><b>RESULTS</b>The total VEGF level was markedly elevated in ROP samples while VEGF 165 b was markedly decreased compared to control group. The relative protein expression level of VEGF 165 b isoform was significantly decreased in ROP patients which were correlated with the ischemia-induced neovascularization.</p><p><b>CONCLUSIONS</b>There was a switch of VEGF splicing from anti-angiogenic to pro-angiogenic family in ROP patients. A specific inhibitor that more selectively targets VEGF 165 and controls the VEGF splicing between pro- and anti-angiogenic families might be a more effective therapy for ROP.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Corpo Vítreo
/
Retinopatia da Prematuridade
/
Recém-Nascido Prematuro
/
Ensaio de Imunoadsorção Enzimática
/
Estudos Prospectivos
/
Isoformas de Proteínas
/
Fator A de Crescimento do Endotélio Vascular
/
Metabolismo
Tipo de estudo:
Estudo observacional
Limite:
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2015
Tipo de documento:
Artigo
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