Simultaneous determination of nine phenolic acids in Salvia miltiorrhiza by RRLC-UV / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a RRLC-UV method for simultaneous determination of nine phenolic acids in Salvia miltiorrhiza.</p><p><b>METHOD</b>3,4-Dihydroxyphenylacetic acid was used as internal standard, and analysis was carried out on an Agilent Zorbax Eclipse Plus C18 (2.1 mm x 50 mm, 1.8 microm) column with acetonitrile-0.1% formic acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.3 mL x min(-1), and the UV detector was monitored at 286 nm.</p><p><b>RESULT</b>All calibration curves showed good linear regression within test ranges (r > or = 0.999 5); and the overall recoveries were in the range of 98.2%-102.6%, with RSD less than 3.1% (n = 3). The overall RSD of precision test were less than 2.4%.</p><p><b>CONCLUSION</b>The developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of S. miltiorrhiza.</p>