Effect of hydrogen inhalation on p38 MAPK activation in rats with lipopolysaccharide- induced acute lung injury / 南方医科大学学报
Journal of Southern Medical University
; (12): 1211-1213, 2012.
Article
em Zh
| WPRIM
| ID: wpr-315500
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hydrogen inhalation on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanisms.</p><p><b>METHODS</b>Thirty-two male SD rats were randomly divided into 4 groups (n=8), namely the normal saline group (SA), saline with 2% hydrogen gas inhalation group (SH group), ALI group, and ALI with hydrogen inhalation group (LH group). In the two ALI groups, ALI was induced by intraperitoneal injection of 15 mg/kg LPS. Treatments with inhalation of 2% hydrogen gas for 6 h was administered after the injection of LPS or saline. The concentrations of tumor necrosis factor-α (TNF-α) in the lung tissue and serum were examined with ELISA. The expression of p38 MAPK in the lung tissue was detected by Western blotting..</p><p><b>RESULTS</b>Hydrogen inhalation decreased the expression of p-p38 MAPK in the lung tissue, and significantly reduced TNF-α content in the lung tissue and serum of rats with ALI.</p><p><b>CONCLUSION</b>Hydrogen inhalation can decrease the expression of TNF-α in the lung tissue and serum, and this effect may be related with reduced p38 MAPK expression and inhibition of p38 MAPK activation.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Sangue
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Administração por Inalação
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Lipopolissacarídeos
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Fator de Necrose Tumoral alfa
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Ratos Sprague-Dawley
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Proteínas Quinases p38 Ativadas por Mitógeno
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Lesão Pulmonar Aguda
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Hidrogênio
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Pulmão
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Metabolismo
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Article