A novel method for studying nuclear localization signal-mediated nuclear translocation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1148-1150, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-315516
ABSTRACT
<p><b>OBJECTIVE</b>To establish a new method for studying the mechanism of nuclear localization signal (NLS)-mediated nuclear translocation in living cells.</p><p><b>METHODS</b>The cells were treated with 67 mg/L 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS), followed by incubation with 1 g/L wheat germ agglutinin (WGA), and their effects on interferon- γ (IFN-γ)-induced nuclear translocation of signal transducer and activator of transcription 1 (STAT1) were observed.</p><p><b>RESULTS</b>Treatment with CHAPS alone had no effect on IFN-γ-induced nuclear translocation of STAT1, while this process was blocked by further WGA incubation.</p><p><b>CONCLUSION</b>We established a new, simple but effective method for studying the mechanism of NLS-mediated nuclear translocation in living cells by perforating the cell membrane with CHAPS treatment.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células HeLa
/
Transdução de Sinais
/
Núcleo Celular
/
Ácidos Cólicos
/
Técnicas Citológicas
/
Interferon gama
/
Sinais de Localização Nuclear
/
Transporte Ativo do Núcleo Celular
/
Fator de Transcrição STAT1
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Artigo
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