Screening of proteins regulated by CCDC19 gene in nasopharyngeal carcinoma using proteomics technology / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1127-1130, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-315521
ABSTRACT
<p><b>OBJECTIVE</b>To screen the proteins under regulation by the candidate tumor suppressor gene CCDC19 using proteomics technology in nasopharyngeal carcinoma (NPC).</p><p><b>METHODS</b>The cellular proteins were extracted from 3D8 NPC cells with CCDC19 overexpression and the control C6 NPC cells. Two-dimensional (2D) gel electrophoresis was employed to compare the protein expression profiles between these two cells, and the differential proteins were identified using peptide mass fingerprinting and database searching. Real-time PCR and Western blotting were used to validate the expression levels of the differential proteins.</p><p><b>RESULTS</b>Matrix-assisted laser desorption/time of flight showed that 3 differential proteins, namely FASN, CTSD and PGK1, were down-regulated by -3.28, -1.64, and -6.97 folds, respectively, which were confirmed by real-time PCR and Western blotting.</p><p><b>CONCLUSION</b>FASN, CTSD and PGK1 are probably the target proteins regulated by CCDC19 in NPC.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA
/
Carcinoma
/
Proteínas
/
Regulação Neoplásica da Expressão Gênica
/
Neoplasias Nasofaríngeas
/
Proteômica
/
Linhagem Celular Tumoral
/
Reação em Cadeia da Polimerase em Tempo Real
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2012
Tipo de documento:
Artigo
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