Effects of laser-assisted cochleostomy on inner hair cell ribbon synapse in rats / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 237-240, 2013.
Article
em Zh
| WPRIM
| ID: wpr-315769
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects on ribbon synapse of inner hair cells after superpulsed CO2 laser-assisted cochleostomy in SD rats.</p><p><b>METHODS</b>Eighteen SD rats were randomly divided into laser-assisted surgery groups (2 W group and 5 W group), sham-operated group and control group. Ten of those were performed a cochleostomy using superpulsed CO2 laser with a corresponding power. Auditory brainstem responses (ABR) were measured pre-and postoperatively. The ribbon synapses at apical and middle cochlear turns were observed under laser scanning confocal microscope and then were quantified with 3ds Max software.</p><p><b>RESULTS</b>The postoperative ABR thresholds of the 2 W and 5 W groups were larger than the preoperative case (t = -5.65, P < 0.01; t = -4.97, P < 0.01). The synapse number at the middle turn decreased significantly in 5 W group (F = 17.15, P < 0.01), while no significant changes were noted at the apical turn (P > 0.05). There was no statistical difference in 2 W group (P > 0.05).</p><p><b>CONCLUSIONS</b>The superpulsed CO2 laser-assisted cochleostomy with high-power is accompanied by a synaptic injury, while no obvious effects after the low-power laser surgery, which might be a safe strategy to preform cochleostomy.</p>
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Índice:
WPRIM
Assunto principal:
Efeitos da Radiação
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Cirurgia Geral
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Sinapses
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Distribuição Aleatória
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Ratos Sprague-Dawley
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Cóclea
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Terapia a Laser
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Lasers de Gás
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Células Ciliadas Auditivas Internas
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2013
Tipo de documento:
Article