Radiobiological characteristics and MRN complex expression of human nasopharyngeal carcinoma cell lines / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 195-199, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-315778
ABSTRACT
<p><b>OBJECTIVE</b>To study the radiobiological characteristic of human nasopharyngeal carcinoma cell lines CNE1 and CNE2 and the changes in expression MRN (Mre11-Rad50-Nbs1) complex in the cell lines exposed to irradiation.</p><p><b>METHODS</b>CNE1 and CNE2 were irradiated by a linear accelerator. Radiobiological characteristics were detected by colony assay and MTT assay. MRN complex expression were examined by Western blot.</p><p><b>RESULTS</b>Surviving fraction at 2 Gy (SF2), quasi-threshold Dose (Dq), and mean lethal dose (Do) of CNE1 were 0.56, 1.449 Gy and 1.480 Gy; SF2, Dq, and Do of CNE2 were 0.44, 0.776 Gy and 1.685 Gy, respectively. Survival fraction of CNE1 at the day 6 after 4 Gy irradiation was 0.59 and that of CNE2 was 0.79 when compared with control, with the up-regulated expressions of Rad50 in CNE1 and Mre11, Rad50 and Nbs1 in CNE2 (P < 0.05).</p><p><b>CONCLUSIONS</b>CNE1 and CNE2 were sensitive to radiation, but there were radioresistance cells in CNE2. The expressions of some components of MRN complex were up-regulated to repair DNA lesions induced by radiation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Efeitos da Radiação
/
Tolerância a Radiação
/
Radioterapia
/
Proteínas Nucleares
/
Carcinoma
/
Regulação Neoplásica da Expressão Gênica
/
Neoplasias Nasofaríngeas
/
Proteínas de Ciclo Celular
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS