Increased invasion ability mechanism of salivary adenoid cystic carcinoma through elevated interstitial fluid pressure in vitro / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 9-12, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-315885
ABSTRACT
<p><b>OBJECTIVE</b>Through a simulation of interstitial fluid pressure (IFP), we developed an in vitro model to explore the change law of biological characteristics of adenoid cystic carcinoma (ACC) under different IFP.</p><p><b>METHODS</b>A pressure cooker was refitted into a controllable pressure device. Cultured ACC-2 cells were subdivided into different groups, namely, negative control (untreated ACC-2) and experimental group (stressed for 3, 6, 12, 24 h under pressure of 7.551, 7.649, 7.747 kPa). CCK-8 and immunofluorescence of Ki67 were used to reflect proliferation ability. Transwell chamber assay was performed to observe the invasion ability of cells.</p><p><b>RESULTS</b>The proliferation ability was positively correlated with treatment time, and the peak value was obtained after the cells were subjected to 7.649 kPa of stress for 24 h. The invasion ability of ACC-2 cells was upregulated under stress.</p><p><b>CONCLUSION</b>We successfully developed an in vitro model of IFP and found that high IFP can stimulate cell proliferation ability and upregulate invasion ability.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas In Vitro
/
Neoplasias das Glândulas Salivares
/
Carcinoma Adenoide Cístico
/
Líquido Extracelular
/
Proliferação de Células
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2014
Tipo de documento:
Artigo
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