Studies on extraction, isolation and composition of Lycium barbarum polysaccharides / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the extraction, isolation and composition of Lycium barbarum polysaccharides (LBP).</p><p><b>METHOD</b>LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc.</p><p><b>RESULT</b>Pure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids.</p><p><b>CONCLUSION</b>LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.</p>