The effect of soy isoflavones on ATP binding cassette A1 expression level in rats without ovaries with atherosclerotic plaque / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 895-899, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-316095
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of soy isoflavones (SI) on ATP binding cassette A1 (ABCA1) expression in rats without ovary with atherosclerosis.</p><p><b>METHODS</b>After they were raised for a week by given basic feed, the ovaries of 50 12-week old SPF rats were removed. The rats were randomly divided into groups by weight. Ten rats were selected as the basic control group (A) that basic feed was given all through the research. The other 40 rats were given high-fat diets and at the end of 4 weeks, these rats were randomly divided into four groups by blood lipid level atherosclerotic model group (B) that was given high-fat diets through the research, low isoflavones group (C) that was given high-fat diets plus 30 mg/kg SI, middle isoflavones group (D) that was given high-fat diets plus 90 mg/kg SI, and high isoflavones group (E) that was given high-fat diets plus 270 mg/kg SI. After 22 weeks, all rats were executed to measure morphological change on the aorta wall, assessing the ABCA1 gene expression in aorta wall by real-time PCR and protein expression by Western blotting in aorta wall, small intestine and liver.</p><p><b>RESULTS</b>Serum lipid level of A, B, C, D, E groups TC levels were (6.82 +/- 0.22), (15.73 +/- 1.51), (10.77 +/- 1.12), (9.95 +/- 1.18), (9.11 +/- 1.12) mmol/L respectively (F = 72.882, P < 0.01); TG levels were (2.49 +/- 0.24), (0.78 +/- 0.13), (0.39 +/- 0.08), (0.29 +/- 0.09), (0.24 +/- 0.09) mmol/L respectively (F = 378.515, P < 0.01); LDL-C levels were (1.29 +/- 0.08), (14.76 +/- 1.23), (8.18 +/- 0.80), (7.85 +/- 0.72), (7.16 +/- 0.64)mmol/L respectively (F = 320.936, P < 0.01); HDL-C levels were (1.94 +/- 0.18), (1.04 +/- 0.10), (1.55 +/- 0.14), (1.88 +/- 0.17), (2.11 +/- 0.22) mmol/L respectively (F = 49.450, P < 0.01). ABCA1 protein expression in intestine, liver and aorta for A, B, C, E groups in intestine were 96.577 +/- 9.743, 5.218 +/- 2.048, 18.060 +/- 5.179, 54.725 +/- 8.960, respectively (F = 172.272, P < 0.01); ABCA1 protein expression in liver of groups of A, B, C, E were 13.657 +/- 2.397, 1.361 +/- 0.266, 7.069 +/- 2.264, 11.793 +/- 2.515 respectively (F = 37.383, P < 0.01); ABCA1 protein expression in aorta of groups A, B, C, E were 6.756 +/- 1.310, 0.027 +/- 0.006, 0.035 +/- 0.002 and 7.479 +/- 1.520 respectively (F = 91.999, P < 0.01). Compared to basic control group, atherosclerotic plaque could be observed in atherosclerotic model group, and it could be partially reversed by isoflavones addition.</p><p><b>CONCLUSION</b>Soy isoflavones treatment might inhibit atherosclerotic progression by lowering the level of blood lipid and increasing ABCA1 expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ovário
/
Farmacologia
/
Glycine max
/
Sangue
/
Ovariectomia
/
Expressão Gênica
/
Química
/
Ratos Sprague-Dawley
/
Transportadores de Cassetes de Ligação de ATP
/
Aterosclerose
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2009
Tipo de documento:
Artigo
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