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Association between promoter methylation of cyclooxygenase-2 and expression, and precancerous gastric lesions in a high-risk population / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 571-575, 2009.
Artigo em Chinês | WPRIM | ID: wpr-316134
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the relationship between cyclooxygenase-2 (COX-2) methylation and expression, and precancerous gastric lesions.</p><p><b>METHODS</b>Methylation status of COX-2 was evaluated by quantitative denaturing high performance liquid chromatography (DHPLC) in 1201 subjects with different gastric lesions. COX-2 expression was assessed by immunohistochemistry and Helicobacter pylori (H pylori) infection status was determined by 13C-urea breath test (13 C-UBT).</p><p><b>RESULTS</b>The percent of COX-2 methylation was increased steadily with the severity of gastric lesions, showing 10.6% of which with superficial gastritis/chronic atrophic gastritis (SG/CAG), 11.8% with intestinal metaplasia (IM) and 13.8% with indefinite dysplasia/dysplasia (Ind DYS/DYS) (chi2 = 8.312, P = 0.016). Stratified analysis indicated that the percents of COX-2 methylation in subjects with H pylori negative still increased with the severity of gastric lesions,of 8.8% in SG/CAG, 10.6% in IM and 14.1% in Ind DYS/DYS (chi2 = 6.629, P= 0.036). Moreover,the methylated proportion of COX-2 was negatively associated with the expression in gastric lesions, from 13.3% with mild expression to 7.6% with strong expression (chi2 = 10.400, P = 0.015).</p><p><b>CONCLUSION</b>Our findings indicated that COX-2 methylation was significantly associated with precancerous gastric lesions and H pylori infection, suggesting that promoter methylation of COX-2 might play an important role in the progression of gastric lesions.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Gastropatias / China / Epidemiologia / Infecções por Helicobacter / Regiões Promotoras Genéticas / Metilação de DNA / Ciclo-Oxigenase 2 / Mucosa Gástrica / Genética Tipo de estudo: Estudo de etiologia Limite: Adulto / Feminino / Humanos / Masculino País/Região como assunto: Ásia Idioma: Chinês Revista: Chinese Journal of Preventive Medicine Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Gastropatias / China / Epidemiologia / Infecções por Helicobacter / Regiões Promotoras Genéticas / Metilação de DNA / Ciclo-Oxigenase 2 / Mucosa Gástrica / Genética Tipo de estudo: Estudo de etiologia Limite: Adulto / Feminino / Humanos / Masculino País/Região como assunto: Ásia Idioma: Chinês Revista: Chinese Journal of Preventive Medicine Ano de publicação: 2009 Tipo de documento: Artigo