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Quantitative microarray-based DNA methylation analysis of E-cadherin gene promoter in acute leukemia / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 41-44, 2007.
Artigo em Chinês | WPRIM | ID: wpr-316249
ABSTRACT
<p><b>OBJECTIVE</b>To quantitatively detect the methylation of E-cadherin gene 5'-CpG islands in acute leukemia by microarray-based DNA analysis and to briefly discuss the role of microarry for detection of methylation in tumors.</p><p><b>METHODS</b>Bisulfite-modified DNA was used as a template for PCR amplification, resulting in conversion of unmethylated cytosine, but not methylated cytosine, into thymine within CpG islands of interest. Five sets of oligonucleotide probes were designed to fabricate a DNA microarray to detect the methylation changes of E-cadherin gene CpG islands in acute leukemia. By drawing a standard curve to assess the levels of changes in methylation detected in the examined samples.</p><p><b>RESULTS</b>Microarray assay was successfully used to quantitatively detect methylation changes of E-cadherin gene in 5 acute leukemia samples. Varying degree of methylation was detected in five regions and the hypermethylation region was the same. The result was validated by gene sequencing.</p><p><b>CONCLUSION</b>Microarray assay may be applied as an useful tool for mapping methylation changes in multiple CpG loci and for leukemia research. It is more time-saving and labor-saving than gene sequencing and can be used to quantitatively detect changes in methylation with high throughput.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Dados de Sequência Molecular / Sequência de Bases / Leucemia Mieloide Aguda / Caderinas / Regiões Promotoras Genéticas / Ilhas de CpG / Metilação de DNA / Análise de Sequência com Séries de Oligonucleotídeos / Leucemia-Linfoma Linfoblástico de Células Precursoras / Genética Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Dados de Sequência Molecular / Sequência de Bases / Leucemia Mieloide Aguda / Caderinas / Regiões Promotoras Genéticas / Ilhas de CpG / Metilação de DNA / Análise de Sequência com Séries de Oligonucleotídeos / Leucemia-Linfoma Linfoblástico de Células Precursoras / Genética Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2007 Tipo de documento: Artigo