Construction of a eukaryotic expression plasmid of Humanin / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

ABSTRACT

<p><b>OBJECTIVE</b>To construct a eukaryotic expression plasmid pcDNA3.1(-)-Humanin.</p><p><b>METHODS</b>The recombinant plasmid pGEMEX-1-Humanin was digested with restriction endonucleases BamH I and Hind III and the Humanin gene fragments, about 100 bp length, were obtained. Then the Humanin gene fragments were inserted into eukaryotic expression vector pcDNA3.1(-) and the recombinant plasmids pcDNA3.1(-)-Humanin were identified by sequencing.</p><p><b>RESULTS</b>Recombinant plasmid DNA successfully produced a band which had the same size as that of the Humanin positive control. The sequence of recombinant plasmids accorded with the Humnain gene sequence.</p><p><b>CONCLUSIONS</b>A eukaryotic expression plasmid of Humanin was successfully constructed.</p>