Immunogenicity of plasmid DNA and adenoviral vectors encoding HIV-1 subtype B env gene / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 415-417, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-316885
ABSTRACT
<p><b>OBJECTIVE</b>To construct DNA and recombinant adenovirus vector vaccines containing an env gene from the prevalent subtype B strain in China and try to use them for therapeutic and prophylactic vaccines.</p><p><b>METHODS</b>The candidate plasmid DNA vaccine pVR-gp160 and recombinant adenovirus vaccine rAdV-gp160 were constructed separately. BALB/c mice were immunized with these two vaccines in different administration schemes. HIV-1 Gp120-specific cellular responses and antibody levels were detected by ELISPOT and ELISA respectively.</p><p><b>RESULTS</b>DNA vaccine alone and combined vaccines in a DNA prime/rAdV-gp160 boost vaccination regimen induced high level of Gp120-specific cellular responses. While low level of Gp120-specific antibodies were elicited in all groups.</p><p><b>CONCLUSION</b>DNA and rAdV vaccines could efficiently express Gp160 protein and activate specific cellular responses.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Vacinas Sintéticas
/
Anticorpos Anti-HIV
/
Genes env
/
Proteína gp120 do Envelope de HIV
/
Adenoviridae
/
China
/
HIV-1
/
Vacinas contra a AIDS
/
Vacinas de DNA
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2010
Tipo de documento:
Artigo
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