Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 340-342, 2010.
Article
em Zh
| WPRIM
| ID: wpr-316905
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus.</p><p><b>METHODS</b>Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0.2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions.</p><p><b>RESULTS</b>The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0.1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus.</p><p><b>CONCLUSION</b>This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing, which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.</p>
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Assunto principal:
Farmacologia
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Polietilenoglicóis
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Ligação Proteica
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Vírion
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Química
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Proteínas do Envelope Viral
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Microscopia Eletrônica de Transmissão
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Vírus da Influenza A Subtipo H1N1
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Anticorpos Antivirais
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2010
Tipo de documento:
Article