Preparation of vero cell-adapted influenza H5N1 virus strain by genetic reassortment / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 119-121, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-316947
ABSTRACT
<p><b>OBJECTIVE</b>To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Vero cell as a substrate.</p><p><b>METHODS</b>Embryonated specific pathogen-free (SPF) hen's eggs and Vero cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics. The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.</p><p><b>RESULTS</b>A Vero cell-adapted influenza H5N1 virus strain was obtained, and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after (F = 0.857, P > 0.05).</p><p><b>CONCLUSION</b>The Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinação Genética
/
Células Vero
/
Vacinas contra Influenza
/
Chlorocebus aethiops
/
Alergia e Imunologia
/
Virus da Influenza A Subtipo H5N1
/
Genética
/
Anticorpos Antivirais
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2010
Tipo de documento:
Artigo
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