Mitigation of Calcification in Bovine Pericardial Bioprosthesis after Amino Acids Posttreatment / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 131-135, 2003.
Artigo
em Coreano
| WPRIM
| ID: wpr-31709
ABSTRACT
BACKGROUND:
Bovine pericardium fixed in glutaraldehyde solution (GA) has been one of the most popular surgical bioprosthesis, however, late calcific degeneration after implantation remains to be solved. To mitigate calcific degeneration, we posttreated the bovine pericardium with amino acids after GA fixation. MATERIAL ANDMETHOD:
40 small pieces of bovine pericardia were fixed in 0.625% GA solution with 4 g/L MgCl2 6H2O as a control group (group 1). 40 pieces fixed in the same GA solution were posttreated with 2% chitosan solution (group 2) and the other 40 pieces posttreated with 8% glutamate (group 3). These were implanted into the belly of forty Fisher 344 rats subdermally and extracted at 1 month, 2 months, 3 months and 4 months after implantation.RESULT:
With atomic absorption spectrophotometry we measured the deposited calcium amount and the results were as follows; 2.01+/-0.13 mg/g in group 1, 2.34+/-0.73 mg/g in group 2, 2.49+/-0.15 mg/g in group 3 at 1 month after implantation, and 3.57+/-0.15 mg/g in group 1, 3.52+/-0.92 mg/g in group 2, 3.46+/-0.12 mg/g in group 3 at the second month. But 5.45+/-0.42 mg/g in group 1, 3.22+/-1.31 mg/g in group 2 and 4.20+/-0.55 mg/g in group 3 at the 3rd month, which have statistical significance in group 2 (p<0.05). Finally at 4th month, 6.01+/-1.21 mg/g in group 1, 3.78+/-1.82 mg/g in group 2, 3.92+/-0.92 mg/g in group 3, which also have statistical significance (p<0.05).CONCLUSION:
This means posttreatment with 2% chitosan shows meaningful calcium mitigation effects after 3rd month on subcutaneously implanted bovine pericardium in the rat models but 8% glutamate shows mitigation effect after 4months in this experiment.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pericárdio
/
Espectrofotometria Atômica
/
Transplante
/
Bioprótese
/
Cloreto de Magnésio
/
Cálcio
/
Glutaral
/
Ácido Glutâmico
/
Modelos Animais
/
Quitosana
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2003
Tipo de documento:
Artigo
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