Effects of Phorbol-12,13-dibuterate on Sodium Currents and Potassium Currents in Rat Trigeminal Ganglion Neurons / 华中科技大学学报（医学）（英德文版）

ABSTRACT

The effects of phorbol-12,13-dibuterate (PDBu) on total sodium current (INa-total), tetrodotoxin-resistant sodium current (INa-TTXr), 4-AP-sensitive potassium current (IA) and TEA-sensitive potassium current (IK) in trigeminal ganglion (TG) neurons were investigated.Whole-cell patch clamp techniques were used to record ion currents in cultured TG neurons of rats. Results revealed that 0.5 μmol/L PDBu reduced the amplitude of INa-total by (38.3±4.5)% (n=6, P＜0.05), but neither the G-V curve (control V0.5 =-17.1±4.3 mV, k=7.4±1.3; PDBu V0.5=-15.9±5.9 mV, k=5.9±1.4; n=6, P＞0.05) nor the inactivation rate constant (control 3.6±0.9 ms; PDBu 3.6±0.8 ms; n=6, P＞0.05) was altered. 0.5 μmol/L PDBu could significantly increase the amplitude of INa-TTXr by (37.2± 3.2)% (n=9, P＜0.05) without affecting the G-V curve (control V0.5=-14.7±6.0 mV, k=6.9±1.4; PDBu V0.5=-11.1±±5.3 mV, k=8.1±1.5; n=5, P＞0.05) or the inactivation rate constant (control 4.6±±0.6 ms; PDBu 4.2±0.5 ms; n=5, P＞0.05). 0.5 μmol/L PDBu inhibited IK by (15.6±5.0) % (n=16, P＜0.05), and V0.5 was significantly altered from - 4.7±1.4 mV to -7.9 ±1.8 mV (n=16, P＜0.05). IA was not significantly affected by PDBu, 0.5 μmol/L PDBu decreased IA by only (0.3±3.2)% (n=5, P＞0.05). It was concluded that PDBu inhibited INa-total but enhanced INa-TTXr, and inhibited IK without affecting IA. These data suggested that the activation of PKC pathway could exert the actions.