Effects of different frequency microvibrations in the vascular endothelial growth factor expression and permeability of vascular endothelial cell / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 136-139, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-317716
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed to evaluate the vascular endothelial growth factor (VEGF) expression and permeability of vascular endothelial cell under microvibration.</p><p><b>METHODS</b>Human umbilical vein endothelial cell (HUVEC) were cultured, randomly vibrated under low frequency of 0.2, 0.5, 2, 5 Hz, 30 min per day. The VEGF mRNA level was detected by Tagman probe real-time fluorescence quantitative polymerase chain reaction (PCR), and the VEGF protein expression level was detected by Western blot. The permeability of vascular endothelial cell was evaluated.</p><p><b>RESULTS</b>Compared with the blank control group, the mRNA and protein expression level of VEGF were significantly increased under 0.2, 0.5 Hz thelial, and increase the permeability microvibration (P<0.05), and decreased under 2, 5 Hz microvibration (P<0.01). The vascular endothelial permeability in creased under 0.2, 0.5 Hz microvibration (P<0.01), whereas the permeability decreased under 2, 5 Hz microvibration (P<0.01).</p><p><b>CONCLUSION</b>0.2-0.5 Hz microvibration can up-regulate the expression of VEGF mRNA and protein in vascular endothelial, and increase the permeability.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Permeabilidade
/
Fisiologia
/
Cordão Umbilical
/
RNA Mensageiro
/
Permeabilidade Capilar
/
Endotélio Vascular
/
Regulação para Cima
/
Química
/
Western Blotting
/
Reação em Cadeia da Polimerase
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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