Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 468-473, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-317783
ABSTRACT
<p><b>OBJECTIVE</b>This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).</p><p><b>METHODS</b>Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.</p><p><b>RESULTS</b>Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.</p><p><b>CONCLUSIONS</b>Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteogênese
/
Ligamento Periodontal
/
Benzofuranos
/
Osteocalcina
/
Diferenciação Celular
/
Células Cultivadas
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2016
Tipo de documento:
Artigo
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