Adenoviral-mediated APE/Ref-1 expression protects rat spiral ganglion cells from oxidative damage / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 773-777, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-317822
ABSTRACT
<p><b>OBJECTIVE</b>To address the question if apurinic/apyrimidinic endonuclease/redox factor 1 (APE/Ref-1) involved in preventing spiral ganglion cells oxidative damage after oxidative stress.</p><p><b>METHODS</b>Primary cultured rat spiral ganglion cells were infected with the adenovirus containing APE/Ref-1 for 48 h, then treated with H2O2 (0, 10, 25, 50, 100, 300 micromol/L) for 1 h, and finally changed back into normal medium. Western blot were used to detect the level of APE/Ref-1 protein in the infected cells to ensure APE/Ref-1 over expression as a result of adenovirus infection. The cell viability was determined by MTT and the apoptosis of spiral ganglion cells was determined by terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).</p><p><b>RESULTS</b>Western blot showed that infection of adenovirus resulted in APE/Ref-1 over expression in the spiral ganglion cells. Over expression of APE/Ref-1 significantly improved cell viability in cultures treated with different concentration H2O2 from 50 to 300 micromol/L However, the apoptosis of cells was significantly inhibited.</p><p><b>CONCLUSIONS</b>Over expression of APE/Ref-1 could protect spiral ganglion cells from oxidative damage.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oxirredução
/
Patologia
/
Farmacologia
/
Técnicas In Vitro
/
Adenoviridae
/
Gânglio Espiral da Cóclea
/
Ratos Sprague-Dawley
/
Apoptose
/
Estresse Oxidativo
/
DNA Liase (Sítios Apurínicos ou Apirimidínicos)
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo
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