Expression of partial tandem duplication of mixed lineage leukaemia in patients with acute leukaemia and their relatives / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 284-289, 2014.
Artigo
em Inglês
| WPRIM
| ID: wpr-317999
ABSTRACT
<p><b>BACKGROUND</b>Partial tandem duplication of mixed lineage leukaemia (MLL-PTD) is detected both in patients with acute leukemia and in healthy people. However, MLL-PTD in relatives of patients with MLL-PTD has not been reported. The objective of this study was to investigate the expression of MLL-PTD in patients with acute leukemia and in their relatives.</p><p><b>METHODS</b>The bone marrow or peripheral blood was collected from patients with acute leukaemia and their relatives. Nested reverse transcription-polymerase chain reaction (RT-PCR) was applied to detect the mRNA expression of the MLLPTD fused gene, and further confirm in genomic DNA level.</p><p><b>RESULTS</b>Analysing MLL-PTD in case 1, the patient's older brother and his younger brother were positive, while his mother and his son were negative. The exon type in case 1 was e9/3 fusion, but in his older brother, it was e9/3 and e11/3 fusion, and in his younger brother, it was e9/3, e10/3, and e11/3 fusion. MLL-PTD in case 2 was negative, but in the patient's older sister was positive, and the exon type was e9/3, e10/3, and e11/3.</p><p><b>CONCLUSIONS</b>The expression of MLL-PTD was present in cases with acute leukaemia with a single expression type. However, various expression types were detected in their healthy relatives. MLL-PTD can couple with other chromosome aberrations, and its impact on disease prognosis remains to be studied further.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Leucemia Mieloide Aguda
/
Doença Aguda
/
Aberrações Cromossômicas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Proteína de Leucina Linfoide-Mieloide
/
Genética
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2014
Tipo de documento:
Artigo
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