Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells.</p><p><b>METHODS</b>Using two types of human macrophage cell lines THP-1 and U937, the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy.</p><p><b>RESULTS</b>SFTSV infected macrophage cell lines THP-1 and U937. Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region.</p><p><b>CONCLUSION</b>The results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.</p>