A study on surface plasmon resonance-based gene chip / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1415-1419, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-318139
ABSTRACT
The surface plasmon resonance (SPR)-based gene chip was prepared according to the following processes First, a film of nanogold, which was synthesized by using Frens' method, was plated on chip by Chlorauric acid/hydroxylamine method. Then probes were fixed on nanogold film by Self-assembled monolayer (SAM) technology. Subsequently, the fixing time and concentration of probes, the sensitivity and the specificity of the chip were optimized. Our results suggested that the chip plated with 2.5 nm nanogold film has a better SPR reflection, and when fixed by probes for 4.5 h at the concentration of 1 500 nmol/L, the gene chip also shows a fine performance of detection and can identify accurately the mismatch between bases in SPR detection system. The gene chip constructed in the research can be used for SPR sensor detection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Química
/
Sensibilidade e Especificidade
/
Ressonância de Plasmônio de Superfície
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Nanopartículas
/
Genética
/
Ouro
/
Métodos
/
Neisseria gonorrhoeae
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2008
Tipo de documento:
Artigo
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