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The neuroprotective role of brain-derived neurotrophic factor for embryonic rat cortical neurons against hypoxia via CREB phosphorylation / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 1377-1380, 2008.
Artigo em Chinês | WPRIM | ID: wpr-318147
ABSTRACT
Transcription factor cyclic AMP response element-binding protein (CREB) in embryonic cortical neurons is an important modulator of Brain-derived neurotrophic factor (BDNF) induced gene expression. Meanwhile, our early researches have indicated that BDNF possesses neuroprotective role for hypoxic neurons against hypoxia. In order todisclose whether the neuroprotective role of BDNF for embryonic rat cortical neurons against hypoxia is fulfilled via nucleoprotein CREB phosphorylation, we used western blotting method to detect the expression of CREB and phosphorylated CREB in experimental groups (with BDNF) and hypoxic control group (without BDNF) with the time changes of exposure to hypoxia. Results indicated that hypoxia and BDNF both could induce phosphorylation of CREB in embryonic cortical neurons. Phosphorylation of CREB in experimental group (with BDNF) was much higher than that in hypoxic control group at the same time points (P<0.01). The expression of phosphorylated CREB reached the highest level at the first hour after being exposed to hypoxia in experimental groups, then phosphorylated CREB decreased slowly and remained at the level for much longer time in experimental groups than in control group. The total amount of CREB in embryonic cortical neurons at the first 0-3 hours after being exposed to hypoxia in experimental groups were the same as that in hypoxic control group. CREB decreased more quickly in hypoxic control group at 5-6 hours after hypoxia. This in vitro research demonstrates that BDNF plays its neuroprotective role for embryonic rat cortical neurons against hypoxia via CREB phosphorylation.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fosforilação / Hipóxia Celular / Células Cultivadas / Córtex Cerebral / Química / Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico / Fármacos Neuroprotetores / Fator Neurotrófico Derivado do Encéfalo / Biologia Celular Limite: Animais Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2008 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Fosforilação / Hipóxia Celular / Células Cultivadas / Córtex Cerebral / Química / Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico / Fármacos Neuroprotetores / Fator Neurotrófico Derivado do Encéfalo / Biologia Celular Limite: Animais Idioma: Chinês Revista: Journal of Biomedical Engineering Ano de publicação: 2008 Tipo de documento: Artigo